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Introduction for the Sample Preparation

Tags:sample preparation, creative proteomics Introduction for the Sample Preparation Summary: Sample preparation is critical for the protein analysis, and Creative Proteomics offers sample preparation service. And whatever kinds of services you want, we can meet your all demands. In this article, we mainly introduce the function and its original theory of this service--sample preparation. And if you are in need of this, we will appreciate your visit. Body: the purpose of proteomics is to study dynamically changing proteins which is expressed by a whole organism, specific tissue or cellular compartment under some conditions. Finally, the main goals of proteomics study are to identify proteins, which are derived from complex mixture and this complex mixture is extracted from cells, and to quantify expression levels of those identification proteins. These protein source is comprised of many more components than the proteins themselves, these non-protein source: nucleic acids, lipids, sugars, and a wide variety of small molecules. What’s more, unlike DNA and RNA, the two are chemically homogeneous, proteins are very heterogeneous and changing widely in their size, charge, hydrophobicity, and structure. In addition, many proteins undergo post-translational modification and they also may have a lot of non-peptide moieties bound to them. That is why the isolating proteins extremely challenging. The sample preparation is the most important step in the whole proteomics analysis. And there are a few methods. In order to avoid the protein losses, the sample has to be kept in a minimum; we have to try our best to keep the sample cold, so that it can avoid the protein modifications and to prevent enzyme activities; therefore, the time of preparation should be very shout and the losses and modifications can be avoided successfully. All aggregates and complexes should be disrupted; disturbing compounds should be removed but the removing proteins should be left. And one point should be noticed that there are not only one possible procedure to treat a sample, and a 100% representation of the proteins, which are included in a cell, will never be obtained in practice. We often chose the method which can express the highest number of different proteins. So that all steps of the sample preparation works. And Creative Proteomics also can provide many other methods for you, and of course we will introduce the best one for you. About the author: Creative Proteomics is a company which can provide the services of the proteomics division, and we also offer our customers all kinds of services which can work very well in labs and scientist’s projects. If you are trying to get some help for your study, and you can contact us or visit our website, and your coming will get the warmest welcome. Links: http://www.creative-proteomics.com/Services/Sample-preparation.htm http://www.creative-proteomics.com
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